Super-resolution 3D microscopy of live whole cells using structured illumination. Super-resolution video microscopy of live cells by structured illumination.
Super-resolution structured illumination microscopy.
Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy. Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination. Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. Laterally modulated excitation microscopy: improvement of resolution by using a diffraction grating. Method of obtaining optical sectioning by using structured light in a conventional microscope. The benefits of the proposed approaches are demonstrated on focal adhesion and tubulin samples in two and three dimensions, and on nanofabricated fluorescent test patterns. This trade-off can be partly overcome by further notch filtering but at the expense of a decrease in signal-to-noise ratio. The new reconstructions point to a trade-off between contrast and a natural noise appearance. Both methods eliminate ad hoc user-adjustable reconstruction parameters in favor of physical parameters, enhancing objectivity. True-Wiener-filtered SIM optimizes contrast given the available signal-to-noise ratio, and flat-noise SIM fully overcomes the structured noise artifact while maintaining resolving power. Here we present a physically realistic noise model that explains the structured noise artifact, which we then use to motivate new complementary reconstruction approaches.
Standard reconstruction algorithms, however, are prone to generate noise-specific artifacts that limit their applicability for lower signal-to-noise data. Once the lock strength rises to be above a certain level, the plug-in automatically sets the nominal frequency to the frequency of the peak found.Super-resolution structured illumination microscopy (SIM) has become a widely used method for biological imaging. By default the plug-in assumes Auto mode, continuously hunting either side of the nominal frequency for a peak profile. The DeBuzzer has three modes, Fast, Auto and Freeze. In the same way as you can tune a guitar, as you home into the fundamental, the beat frequency between the tone and the fundamental will become slower and slower the closer you get. The third method of isolating the fundamental frequency is a tone generator. It will automatically mark the three highest peaks found, and you can set the nominal frequency by clicking one of the peaks on the graph. Second is the large FFT display which accentuates any frequency components that are consistently present, making them stand out in the plot. The DeBuzzer has several ways of helping isolate the fundamental frequency.įirst is the Peak Profile that is displayed above the central rotary control, which shows the strength of a given nominal frequency in the input signal.
For this process to work well, the fundamental frequency must be very accurately determined. The comb filter has many tight notches that eliminate the fundamental frequency and associated harmonics of the buzz. The Sonnox Oxford DeBuzzer is divided into two stages: Detection and Removal.īuzz removal is usually accomplished using a comb filter.
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